ESAM supports neutrophil extravasation, activation of Rho, and VEGF-induced vascular permeability

نویسندگان

  • Frank Wegmann
  • Björn Petri
  • Alexander Georg Khandoga
  • Christian Moser
  • Andrej Khandoga
  • Stefan Volkery
  • Hang Li
  • Ines Nasdala
  • Oliver Brandau
  • Reinhard Fässler
  • Stefan Butz
  • Fritz Krombach
  • Dietmar Vestweber
چکیده

Endothelial cell-selective adhesion molecule (ESAM) is specifically expressed at endothelial tight junctions and on platelets. To test whether ESAM is involved in leukocyte extravasation, we have generated mice carrying a disrupted ESAM gene and analyzed them in three different inflammation models. We found that recruitment of lymphocytes into inflamed skin was unaffected by the gene disruption. However, the migration of neutrophils into chemically inflamed peritoneum was inhibited by 70% at 2 h after stimulation, recovering at later time points. Analyzing neutrophil extravasation directly by intravital microscopy in the cremaster muscle revealed that leukocyte extravasation was reduced (50%) in ESAM(-/-) mice without affecting leukocyte rolling and adhesion. Depletion of >98% of circulating platelets did not abolish the ESAM deficiency-related inhibitory effect on neutrophil extravasation, indicating that it is only ESAM at endothelial tight junctions that is relevant for the extravasation process. Knocking down ESAM expression in endothelial cells resulted in reduced levels of activated Rho, a GTPase implicated in the destabilization of tight junctions. Indeed, vascular permeability stimulated by vascular endothelial growth factor was reduced in ESAM(-/-) mice. Collectively, ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall, possibly by influencing endothelial cell contacts.

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عنوان ژورنال:
  • The Journal of Experimental Medicine

دوره 203  شماره 

صفحات  -

تاریخ انتشار 2006